Functionalized Micromachines for Selective and Rapid Isolation of Nucleic Acid Targets from Complex Samples


Kagan D., Campuzano S., Balasubramanian S., Kuralay F. , Flechsig G., Wang J.

NANO LETTERS, vol.11, no.5, pp.2083-2087, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.1021/nl2005687
  • Title of Journal : NANO LETTERS
  • Page Numbers: pp.2083-2087

Abstract

The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for "on-the-fly" hybridization and selective single-step isolation of target nucleic acids from "raw" microliter biological samples (serum, urine, crude E. coli lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.