Ciprofloxacin-induced cytotoxicity and apoptosis in HeLa cells
TOXICOLOGY MECHANISMS AND METHODS, cilt.15, sa.5, ss.339-342, 2005 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 5
- Basım Tarihi: 2005
- Doi Numarası: 10.1080/153765291009877
- Dergi Adı: TOXICOLOGY MECHANISMS AND METHODS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.339-342
- Anahtar Kelimeler: apoptosis, ciprofloxacin, cytotoxicity, HeLa cells, HUMAN-IMMUNODEFICIENCY-VIRUS, METAL-LINKED DIMER, TAT PROTEIN, CASPASE ACTIVATION, MITOCHONDRIAL-DNA, FREE-RADICALS, IN-VITRO, ZINC, GROWTH, PROLIFERATION
- Hacettepe Üniversitesi Adresli: Evet
Özet
The aim of this study was to investigate the possible cytotoxic and apoptotic effects of a fluoroquinolone (FQ) antibiotic, ciprofloxacin (CPFX), on HeLa and HeLa-tat cell lines. The cultured cells were incubated with three different (20 to 100 mg/L) concentrations of CPFX, and cytotoxicity was determined by trypan blue test. A dose- and time-dependent decrease in cell proliferation was observed for both cell types except 50 to 100 mg/L CPFX for 24 h for HeLa-tat cells. CPFX-induced apoptosis was also measured by the acridin orange-ethidium bromide staining using a fluorescence microscope in both cell types. Our results showed an induction of apoptosis at 100 mg/L concentration of CPFX after 24 and 48 h of incubation in both cell types. These data confirmed our previous studies obtained with normal human fibroblast cell cultures and indicated that CPFX induces cytotoxicity and apoptosis in HeLa cells.