Ciprofloxacin-induced cytotoxicity and apoptosis in HeLa cells

Gurbay A., OSMAN M., FAVIER A., HINCAL F.

TOXICOLOGY MECHANISMS AND METHODS, cilt.15, sa.5, ss.339-342, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1080/153765291009877
  • Dergi Adı: TOXICOLOGY MECHANISMS AND METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.339-342
  • Anahtar Kelimeler: apoptosis, ciprofloxacin, cytotoxicity, HeLa cells, HUMAN-IMMUNODEFICIENCY-VIRUS, METAL-LINKED DIMER, TAT PROTEIN, CASPASE ACTIVATION, MITOCHONDRIAL-DNA, FREE-RADICALS, IN-VITRO, ZINC, GROWTH, PROLIFERATION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the possible cytotoxic and apoptotic effects of a fluoroquinolone (FQ) antibiotic, ciprofloxacin (CPFX), on HeLa and HeLa-tat cell lines. The cultured cells were incubated with three different (20 to 100 mg/L) concentrations of CPFX, and cytotoxicity was determined by trypan blue test. A dose- and time-dependent decrease in cell proliferation was observed for both cell types except 50 to 100 mg/L CPFX for 24 h for HeLa-tat cells. CPFX-induced apoptosis was also measured by the acridin orange-ethidium bromide staining using a fluorescence microscope in both cell types. Our results showed an induction of apoptosis at 100 mg/L concentration of CPFX after 24 and 48 h of incubation in both cell types. These data confirmed our previous studies obtained with normal human fibroblast cell cultures and indicated that CPFX induces cytotoxicity and apoptosis in HeLa cells.