Ciprofloxacin-induced cytotoxicity and apoptosis in HeLa cells


Gurbay A. , OSMAN M., FAVIER A., HINCAL F.

TOXICOLOGY MECHANISMS AND METHODS, vol.15, no.5, pp.339-342, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 5
  • Publication Date: 2005
  • Doi Number: 10.1080/153765291009877
  • Title of Journal : TOXICOLOGY MECHANISMS AND METHODS
  • Page Numbers: pp.339-342

Abstract

The aim of this study was to investigate the possible cytotoxic and apoptotic effects of a fluoroquinolone (FQ) antibiotic, ciprofloxacin (CPFX), on HeLa and HeLa-tat cell lines. The cultured cells were incubated with three different (20 to 100 mg/L) concentrations of CPFX, and cytotoxicity was determined by trypan blue test. A dose- and time-dependent decrease in cell proliferation was observed for both cell types except 50 to 100 mg/L CPFX for 24 h for HeLa-tat cells. CPFX-induced apoptosis was also measured by the acridin orange-ethidium bromide staining using a fluorescence microscope in both cell types. Our results showed an induction of apoptosis at 100 mg/L concentration of CPFX after 24 and 48 h of incubation in both cell types. These data confirmed our previous studies obtained with normal human fibroblast cell cultures and indicated that CPFX induces cytotoxicity and apoptosis in HeLa cells.