The effect of hydrogen peroxide/colloidal silver on reducing the colonization and growth of heterotrophic bacteria in dental unit waterlines

OZALPL M., Bulut O. E. , ATAÇ A. S. , EKİZOĞLU M. , KART D. , ÇELİK H. H. , ...Daha Fazla

TURKISH JOURNAL OF BIOLOGY, cilt.37, ss.336-341, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 37 Konu: 3
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3906/biy-1204-23
  • Sayfa Sayıları: ss.336-341


The objective of the present study was to investigate reduction in the colonization and growth of heterotrophic bacteria in dental unit waterlines by using hydrogen peroxide/colloidal silver as disinfectant. Twenty-seven dental units were included; 6 units that were more than 20 years old and 7 units that had been in use for 2 years comprised the old and new treatment groups, respectively. Fourteen units served as controls. The treatment groups were disinfected continuously and every 4 weeks shock doses were applied over a 20-week period. Water samples were taken before treatment, 1 and 2 weeks after treatment, and thereafter every 4 weeks; then they were inoculated onto R2A agar plates. While 1-16-week results for the old treatment group showed total heterotrophic bacterial counts of higher than 1 x 10(5) cfu/mL, at 20 weeks they were below 7.5 x 10(2) cfu/mL. Only 2 units were able to reach levels of <= 200 cfu/mL, which is the dental unit water quality standard. For the new treatment group it was achieved for all units after 1 week. Electron microscopic analysis also revealed that while biofilm formation was more evident in the old treatment group, after a longer treatment period biofilms were eliminated completely. The findings indicate that disinfection was effective in improving the output water quality using hydrogen peroxide/colloidal silver disinfectant.