Possible Effect of Chelation Treatment on Metabolomic and Lipidomic Analysis in Lead Exposure
JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, cilt.64, sa.5, 2022 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 64 Sayı: 5
- Basım Tarihi: 2022
- Doi Numarası: 10.1097/jom.0000000000002503
- Dergi Adı: JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, CINAHL, EMBASE, Environment Index, MEDLINE, Pollution Abstracts, Public Affairs Index
- Anahtar Kelimeler: chelation, lead, lipidomic, metabolomic, oxysterol, TOXICITY, DISEASE, ACID, ANTIOXIDANTS, MECHANISMS, DAMAGE
- Hacettepe Üniversitesi Adresli: Evet
Özet
Objective: This study aimed to examine patients with lead poisoning in terms of metabolomic profiles and bioactive lipids (oxysterols and sphingosine 1-phosphate [S1P]) before and after chelation therapy. Methods: Consent was obtained from 42 individuals diagnosed with lead poisoning and blood and urine samples were collected before and after chelation therapy. The levels of 7-ketocholesterol (7-KC), cholestan-3b,5a,6b-triol (Ctriol), and S1P were measured via LC-MS/MS. Metabolomic analysis was performed via GC-MS. Results: 7-KC and C-triol levels were detected higher before chelation therapy compared with after therapy (P < 0.001 for both). S1P levels were measured higher before the therapy. The results also showed that sphingolipid metabolism-related pathways were affected by lead toxicity as well as other related pathways. Conclusion: This preliminary study showed that lipid metabolism is affected in lead exposure and chelation therapy is effective in reversing possible damage.