Total phenolic content, cyclooxygenases, alpha-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits


Barut B., ŞÖHRETOĞLU D.

JOURNAL OF RESEARCH IN PHARMACY, vol.24, no.5, pp.623-631, 2020 (Journal Indexed in ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 5
  • Publication Date: 2020
  • Doi Number: 10.35333/jrp.2020.217
  • Title of Journal : JOURNAL OF RESEARCH IN PHARMACY
  • Page Numbers: pp.623-631

Abstract

The aim of the present study was to investigate total phenolic content and biological effects of methanol extracts from Cornus sanguinea L. leaves (LME) and fruits (FME). Total phenolic contents, COX-1/COX-2, alpha-glucosidase, AChE, tyrosinase inhibitory and DPPH radical scavenging effects of both extracts were investigated by using spectrophotometric methods. The total phenolic contents of LME and FME were determined as 191.14 +/- 4.84 and 31.51 +/- 2.68 mg GAE/g dry weight, respectively. LME inhibited COX-1 enzyme 70.71 +/- 1.88% and 79.38 +/- 0.92% at 50 and 100 mu g/mL. LME had higher COX-1 and COX-2 inhibitory effects than that of FME. LME inhibited alpha-glucosidase stronger than positive control, acarbose. On the other hand, both extracts showed lower AChE inhibition actions compared to positive control, galantamine. Moreover, LME had higher tyrosinase inhibitory effect than FME. Both extracts scavenged DPPH radical in a concentration-dependent manner. Also, LME had stronger scavenging effect than that of FME. To our knowledge, current work is the first report on tyrosinase, AChE, as well as COX-1 inhibitory properties of C. sanguinea. These results suggested that LME of C. sanguinea have a promising potential for the treatment of several disorders but further studies are needed to support the this assumption.