Kinetic properties of glucose-6-phosphate dehydrogenase from lamb kidney cortex


Ulusu N., Tandogan B., Tezcan F.

BIOCHIMIE, cilt.87, sa.2, ss.187-190, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 87 Konu: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.biochi.2004.11.002
  • Dergi Adı: BIOCHIMIE
  • Sayfa Sayıları: ss.187-190

Özet

Glucose-6-phosphate dehydrogenase is the key regulatory enzyme of the pentose phosphate pathway and one of the products of this enzyme; NADPH has a critical role in the defence system against the free radicals. In this study, glucose-6-phosphate dehydrogenase from lamb kidney cortex kinetic properties is examined. The purification procedure is composed of two steps after ultracentrifugation for rapid and easy purification: 2'. 5'-ADP Sepharose 4B affinity and DEAE Sepharose Fast Flow anion exchange chromatography. Previously, we used this procedure for the purification of glucose-6-phosphate dehydrogenase from bovine lens. The double reciprocal plots and product inhibition studies showed that the enzyme obeys 'Ordered Bi Bi' mechanisin: K-m (NADP+) K-m (G-6-P) and Ki (G-6-P) (dissociation constant of the enzyme-G-6-P complex) were found to be 0.018 +/- 0.002, 0.039 +/- 0.006 and 0.029 +/- 0.005 mM, respectively, by using nonlinear regression analysis. The enzyme was stable at 4 degrees C for a week. (c) 2004 Elsevier SAS. All rights reserved.