The purpose of this study was to analyse the relationship between joint position sense and pain in patients with osteoarthritis (OA) and compare the results with age-matched control subjects. Thirty patients between 56 and 72 years with bilateral OA of grade 3 and 4 according to the Kellgren-Lawrence radiographic scores and 26 healthy subjects were included in this study. Pain was evaluated by the Western Ontario and McMaster Osteoarthritis Index (WOMAC) and visual analogue scale (VAS). Joint position sense was analysed, at 30, 60 and 90 degrees of knee flexion, using the motion analysis system designed by us. The relationship between pain intensity and joint position sense, and comparison between patients and controls were analysed. Statistically significant differences were found between OA patients and healthy subjects in joint position sense at 30, 60 and 90 degrees of knee flexion in favour of healthy subjects (p < 0.05). The highest error degree was shown at the angle of 60degrees flexion in both groups. Pain intensity and inaccuracy degrees of knee joint position sense were not correlated at 30degrees knee flexion. These were instead positive correlated at 60degrees and 90degrees knee flexion (p < 0.05). Relationship between pain intensity and knee joint position sense is very important for OA patient in the improvement of rehabilitation programmes.