Cd(II) removal from human plasma by cysteine-immobilized PHEMA microspheres

Bektas S., Disbudak A., Denizli A., Genc O.

TRACE ELEMENTS AND ELECTROLYTES, vol.19, no.1, pp.26-32, 2002 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 1
  • Publication Date: 2002
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.26-32
  • Hacettepe University Affiliated: No


Poly(2-hydroxyethylmethacrylate), PHEMA, microspheres carrying cysteine were prepared and used for the removal of cadmium(II) ions from human plasma. PHEMA microspheres, in the size range of 150-200 mum in diameter, were produced by a modified suspension copolymerization of HEMA and EGDMA. Metal complexing aminoacid ligand cysteine was immobilized onto PHEMA microspheres. The maximum amount of cysteine-immobilized was 0.318 mmol/g. These hydrophilic microspheres with a swelling ratio of 58% (w/w), and carrying cysteine were used in Cd(II) removal studies in a packed-bed column system. The maximum amount of Cd(II) removed from human plasma by cysteine-immobilized PHEMA microspheres was 0.065 mmol/g. Cd(II) ions could be repeatedly adsorbed and desorbed with these novel cysteine-immobilized PHEMA microspheres without noticeable loss in their adsorption capacity.