In the study, it was aimed to examine the effect of the designed generative multimedia learning environment on retention. The one group pretest-posttest design was used in the study. 31 teacher candidates, who were studying in Hacettepe University, Faculty of Education, and Department of Chemistry Education during 2010 - 2011 academic years fall term, were included in the study. Achievement Test about Chemical Bonds and open-ended questions intended for learning teacher candidates' opinions were used in the study as a data collection tool. Paired sample t- test was used to analyze the data. It was found as a result of the study that multimedia learning environment applications caused a retention of learning.