In this paper, we present a very popular and low-cost sensor material as disposable pencil graphite electrode (PGE) system for the trace-level determination of vardenafil hydrochloride (VRL). Electrochemical behavior and determination of VRL was carried out by cyclic and square-wave adsorptive anodic stripping (SW-AdAS) voltammetry. The proposed method was highly sensitive and had a linear calibration range from 3 x 10(-10) to 1 x 10(-7) M with a correlation coefficient of 0.997. The limit of detection and limit of quantitation values were calculated as 1 x 10(-10) and 3 x 10(-10) M, respectively. Developed method with disposable PGE shows high sensitivity, selectivity and stability in a wide concentration range for VRL determination without the necessity of any modification. Additionally, this system was successfully applied in Levitra(A (R)) tablets and spiked human serum in trace level of VRL. Some kinetic parameters of VRL's were represented for the first time. The results showed that the proposed method is highly sensitive, easy and low cost for trace-level determination of VRL.