Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles

Karakoc V., Yilmaz E., TÜRKMEN D., Ozturk N., Akgol S., DENİZLİ A.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.45, sa.2, ss.188-193, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.ijbiomac.2009.04.023
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.188-193
  • Anahtar Kelimeler: Albumin purification, Nanoparticles, Metal-chelates, METAL AFFINITY ADSORPTION, MAGNETIC BEADS, PROTEIN, CHROMATOGRAPHY, PLASMA
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Poly(hydroxyethyl methacrylate) (PHEMA) nanoparticles with an average size of 300 nm in diameter and with a polydispersity index of 1.156 were produced by surfactant free emulsion polymerization. Specific surface area of the PHEMA nanoparticles was found to be 996 m(2)/g. Metal-chelating ligand 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) was covalently attached to the PHEMA nanoparticles. IMEO content was 0.97 mmol IEMC/g. The morphology and properties of these nanoparticles were characterized with scanning electron microscopy, Fourier transform infrared spectroscopy and atomic force microscopy. The Cu2+-chelated PHEMA-IMEO nanoparticles were used in the adsorption-elution studies of human serum albumin (HSA) in a batch system. Maximum HSA adsorption amount of the Cu2+ chelated nanoparticles was 680 mg HSA/g. The PHEMA-IMEO-Cu2+ nanoparticles exhibited a quite high adsorption capacity and fast adsorption rate due to their high specific surface area and the absence of internal diffusion resistance. (C) 2009 Elsevier B.V. All rights reserved.