Direct N- or C-Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach

Cong M., Tavakolpour S., Berland L., Glockner H., Andreiuk B., Rakhshandehroo T., ...Daha Fazla

BIOCONJUGATE CHEMISTRY, cilt.32, sa.11, ss.2397-2406, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 11
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1021/acs.bioconjchem.1c00442
  • Dergi Adı: BIOCONJUGATE CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.2397-2406
  • Hacettepe Üniversitesi Adresli: Hayır

Özet

Site-specific protein labeling is important in biomedical research and biotechnology. While many methods allow site-specific protein modification, a straightforward approach for efficient N-terminal protein labeling is not available. We introduce a novel sortase-mediated swapping approach for a one-step site-specific N-terminal labeling with a near-quantitative yield. We show that this method allows rapid and efficient cleavage and simultaneous labeling of the N or C termini of fusion proteins. The method does not require any prior modification beyond the genetic incorporation of the sortase recognition motif. This new approach provides flexibility for protein engineering and site-specific protein modifications.