Recombinant human granulocyte colony-stimulating factor (rhG-CSF) promotes in vitro platelet aggregation

Kaptan K., Ifran A., Beyan C., Sertkaya D.

HEMATOLOGY, cilt.12, sa.5, ss.441-444, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 5
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/10245330701384286
  • Dergi Adı: HEMATOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.441-444
  • Anahtar Kelimeler: granulocyte colony-stimulating factor, leukopoiesis, blood platelets, platelet activation, thrombosis, ACUTE ARTERIAL THROMBOSIS, POLYMORPHONUCLEAR LEUKOCYTES, NEUTROPHIL ACTIVATION, HEMOSTATIC SYSTEM, RECEPTOR, DONORS, THROMBOPOIETIN
  • Hacettepe Üniversitesi Adresli: Evet

Özet

rhG- CSF is increasingly used for stimulation of granulopoiesis and stem cell mobilization in healthy donors for allogeneic stem cell transplantation. However, a possible association between thrombosis and rhG- CSF administration has been reported. For that reason, in this study, we investigated the effect of rhG- CSF on platelet aggregation in whole blood of 10 healthy volunteers. Three concentrations of rhG-CSF solution (1, 10 and 100 ng/ml) were prepared. Each concentration of rhG-CSF solution and a control diluent without rhG-CSF were incubated with whole blood. Incubation with rhG-CSF solutions would result in 0.1, 1.0 and 10 ng/ml rhG-CSF concentrations in the blood. After incubation, aggregation responses were evaluated with ADP (5 and 10 mu M) and collagen (2 and 5 mu g/ml) in whole blood. When compared to control, preincubation with all dilutions of rhG-CSF augmented aggregation of platelets induced by ADP and collagen in a statistically significant manner (p < 0.05 for all comparisons). There was also a relationship between rhG-CSF concentration (1, 10 and 100 ng/ml) and augmentation of platelet aggregation response (p < 0.0001 for 5 -10 mu M ADP; p < 0.0001 for 2 -5 mu g/ml collagen). In conclusion, this study with an in vitro model showed that rhG-CSF administration may lead to platelet hyperaggregability.