Purpose: The aim of the study was to investigate the acute effects of different types of exercises on hemodynamic responses and fatigue in Duchenne muscular dystrophy (DMD). Methods: Thirty DMD subjects of an early stage were included in the study. One of the three different types of exercises was applied in only one exercise session. These were three-minute stair climbing, 40-minutes cycling and 40-minutes stretching-strengthening and aerobic exercises for the upper and lower extremities accompanied by a physiotherapist. Acute exercise responses including the heart rate, oxygen saturation, muscle strength responses, fatigue and timed-performances were assessed just before and after the exercises. A questionnaire was applied to assess how perceived fatigue reflected to daily activity performance after exercises. Results: The heart rate after climbing stairs and physiotherapy program were increased while expressed fatigue levels after all three exercises were increased (p< 0.05). After the cycling and physiotherapy program, duration of standing up was increased (p< 0.05). There was an increase in muscle strength after climbing stairs and decrease after cycling while there was no significant difference after physiotherapy (p< 0.05). No performance reduction in daily activities after exercises was declared by subjects. Conclusion: Physiotherapy exercises under the supervision of a physiotherapist may be more effective and less tiring compared to other exercises in DMD patients.