Effects of alginate-chitosan core-shell nanoparticles encapsulated in gelatin methacrylate hydrogel on chondrogenesis of mesenchymal stem cells


Aksoy Korpe D., Guler S., AYDIN H. M. , DUMAN M.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2021
  • Doi Number: 10.1080/00914037.2021.2014484
  • Title of Journal : INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Keywords: alginate-chitosan, chondrogenesis, core-shell nanoparticles, gelatin methacrylate, tissue engineering, GROWTH-FACTOR-BETA, PROTEIN DELIVERY, FACTOR-I, RELEASE, MATRIX, DIFFERENTIATION, CHONDROCYTES, MICROSPHERES, TGF-BETA-1

Abstract

In this paper, gelatin methacrylate hydrogel scaffold, functionalized with growth factors loaded alginate-chitosan core-shell nanoparticles, were developed to enhance chondrogenic differentiation of adipose-derived mesenchymal stem cells. With the chosen preparation conditions, the encapsulation efficiency of transforming growth factor beta-1 (TGF beta-1) and insulin-like growth factor-1 (IGF-1) was calculated at 93.34% and 91.92%, respectively. The assay groups which contain loaded nanoparticles in hydrogels exhibited the highest DNA and glycosaminoglycan contents with 2.93 and 573.60 mu g/sample, respectively. Moreover, in the same group, it was observed that the amount of collagen also increased by 3.6-fold. Thus, the functionalized scaffold is promising construct for tissue engineering applications.