Effects of alginate-chitosan core-shell nanoparticles encapsulated in gelatin methacrylate hydrogel on chondrogenesis of mesenchymal stem cells

Aksoy Korpe D., Guler S., AYDIN H. M., DUMAN M.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, cilt.72, sa.5, ss.355-365, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/00914037.2021.2014484
  • Dergi Adı: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Sayfa Sayıları: ss.355-365
  • Anahtar Kelimeler: alginate-chitosan, chondrogenesis, core-shell nanoparticles, gelatin methacrylate, tissue engineering, GROWTH-FACTOR-BETA, PROTEIN DELIVERY, FACTOR-I, RELEASE, MATRIX, DIFFERENTIATION, CHONDROCYTES, MICROSPHERES, TGF-BETA-1
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In this paper, gelatin methacrylate hydrogel scaffold, functionalized with growth factors loaded alginate-chitosan core-shell nanoparticles, were developed to enhance chondrogenic differentiation of adipose-derived mesenchymal stem cells. With the chosen preparation conditions, the encapsulation efficiency of transforming growth factor beta-1 (TGF beta-1) and insulin-like growth factor-1 (IGF-1) was calculated at 93.34% and 91.92%, respectively. The assay groups which contain loaded nanoparticles in hydrogels exhibited the highest DNA and glycosaminoglycan contents with 2.93 and 573.60 mu g/sample, respectively. Moreover, in the same group, it was observed that the amount of collagen also increased by 3.6-fold. Thus, the functionalized scaffold is promising construct for tissue engineering applications.