Effects of alginate-chitosan core-shell nanoparticles encapsulated in gelatin methacrylate hydrogel on chondrogenesis of mesenchymal stem cells
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, cilt.72, sa.5, ss.355-365, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 72 Sayı: 5
- Basım Tarihi: 2023
- Doi Numarası: 10.1080/00914037.2021.2014484
- Dergi Adı: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
- Sayfa Sayıları: ss.355-365
- Anahtar Kelimeler: alginate-chitosan, chondrogenesis, core-shell nanoparticles, gelatin methacrylate, tissue engineering, GROWTH-FACTOR-BETA, PROTEIN DELIVERY, FACTOR-I, RELEASE, MATRIX, DIFFERENTIATION, CHONDROCYTES, MICROSPHERES, TGF-BETA-1
- Hacettepe Üniversitesi Adresli: Evet
Özet
In this paper, gelatin methacrylate hydrogel scaffold, functionalized with growth factors loaded alginate-chitosan core-shell nanoparticles, were developed to enhance chondrogenic differentiation of adipose-derived mesenchymal stem cells. With the chosen preparation conditions, the encapsulation efficiency of transforming growth factor beta-1 (TGF beta-1) and insulin-like growth factor-1 (IGF-1) was calculated at 93.34% and 91.92%, respectively. The assay groups which contain loaded nanoparticles in hydrogels exhibited the highest DNA and glycosaminoglycan contents with 2.93 and 573.60 mu g/sample, respectively. Moreover, in the same group, it was observed that the amount of collagen also increased by 3.6-fold. Thus, the functionalized scaffold is promising construct for tissue engineering applications.