Effect of Allyl Isothiocyanate (AITC) in Both Nitrite- and Nitrosamine-Induced Cell Death, Production of Reactive Oxygen Species, and DNA Damage by the Single-Cell Gel Electrophoresis (SCGE): Does It Have Any Protective Effect on HepG2 Cells?

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Atıf İçin Kopyala

Erkekoglu P., BAYDAR T.

INTERNATIONAL JOURNAL OF TOXICOLOGY, cilt.29, sa.3, ss.305-312, 2010 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 29 Sayı: 3
• Basım Tarihi: 2010
• Doi Numarası: 10.1177/1091581810366313
• Dergi Adı: INTERNATIONAL JOURNAL OF TOXICOLOGY
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.305-312
• Anahtar Kelimeler: nitrite, nitrosamine, AITC, HepG2, genotoxicity, single-cell gel electrophoresis (SCGE), LUNG-CANCER RISK, EPIDEMIOLOGIC EVIDENCE, N-NITROSODIBUTYLAMINE, (SCGE)/HEPG2 ASSAY, G2 ASSAY, CHEMOPREVENTION, CARCINOGENESIS, INHIBITION, TOBACCO, FOOD
• Hacettepe Üniversitesi Adresli: Evet

Özet

The current study was designed to investigate possible protective effect of allyl isothiocyanate (AITC) in nitrite- and nitrosamine-treated human hepatoma cells (HepG2) with the evaluation by cytotoxic effects and genotoxic effects determined by the single-cell gel electrophoresis (SCGE). Allyl isothiocyanate treatment enhanced cell viability and reduced intracellular reactive oxygen species (ROS) production in both nitrite- and nitrosamine-treated cells significantly. In SCGE, when compared to untreated control cells, all of the treated groups caused increases in the tail intensity (%) such as nitrite at 17%, N-nitrosodimethylamine (NDMA) at 279%, N-nitrosodiethylamine (NDEA) at 324%, and N-nitrosomorpholine (NMOR) at 288%. Allyl isothiocyanate reduced the tail intensity caused by nitrite 36%, by NDMA 36%, by NDEA 49%, and by NMOR 32%, respectively, when compared to each individual toxic compound-treated group. In conclusion, AITC protected HepG2 cells against cytotoxic and genotoxic effects caused by nitrite and the nitrosamines.