Coinfections identified from metagenomic analysis of cervical lymph nodes from tularemia patients


Birdsell D. N. , Ozsurekci Y., Rawat A., Aycan A. E. , Mitchell C. L. , Sahl J. W. , ...More

BMC INFECTIOUS DISEASES, vol.18, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18
  • Publication Date: 2018
  • Doi Number: 10.1186/s12879-018-3218-2
  • Title of Journal : BMC INFECTIOUS DISEASES

Abstract

Background: Underlying coinfections may complicate infectious disease states but commonly go unnoticed because an a priori clinical suspicion is usually required so they can be detected via targeted diagnostic tools. Shotgun metagenomics is a broad diagnostic tool that can be useful for identifying multiple microbes simultaneously especially if coupled with lymph node aspirates, a clinical matrix known to house disparate pathogens. The objective of this study was to analyze the utility of this unconventional diagnostic approach (shotgun metagenomics) using clinical samples from human tularemia cases as a test model. Tularemia, caused by the bacterium Francisella tularensis, is an emerging infectious disease in Turkey. This disease commonly manifests as swelling of the lymph nodes nearest to the entry of infection. Because swollen cervical nodes are observed from many different types of human infections we used these clinical sample types to analyze the utility of shotgun metagenomics.