PROTEIN AND PEPTIDE LETTERS, vol.17, no.5, pp.667-674, 2010 (SCI-Expanded)
Article / Article
PROTEIN AND PEPTIDE LETTERS
Science Citation Index Expanded (SCI-EXPANDED), Scopus
Hacettepe University Affiliated:
Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys 'branched' mechanism: Km(NADPH) 18 +/- 3 mu M and Km(GSSG) 65 +/- 5 mu M were determined.