Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase


Tandogan B., Ulusu N. N.

PROTEIN AND PEPTIDE LETTERS, cilt.17, ss.667-674, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 17 Konu: 5
  • Basım Tarihi: 2010
  • Doi Numarası: 10.2174/092986610791112684
  • Dergi Adı: PROTEIN AND PEPTIDE LETTERS
  • Sayfa Sayıları: ss.667-674

Özet

Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys 'branched' mechanism: Km(NADPH) 18 +/- 3 mu M and Km(GSSG) 65 +/- 5 mu M were determined.