Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase


Tandogan B., Ulusu N. N.

PROTEIN AND PEPTIDE LETTERS, vol.17, no.5, pp.667-674, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 5
  • Publication Date: 2010
  • Doi Number: 10.2174/092986610791112684
  • Journal Name: PROTEIN AND PEPTIDE LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.667-674
  • Hacettepe University Affiliated: Yes

Abstract

Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys 'branched' mechanism: Km(NADPH) 18 +/- 3 mu M and Km(GSSG) 65 +/- 5 mu M were determined.