Proof-of-principle for two-stage photodynamic therapy: hypoxia triggered release of singlet oxygen

Ayan S., GÜNAYDIN G. , Yesilgul-Mehmetcik N., Gedik M. E. , Seven O., Akkaya E. U.

CHEMICAL COMMUNICATIONS, vol.56, no.94, pp.14793-14796, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 94
  • Publication Date: 2020
  • Doi Number: 10.1039/d0cc06031c
  • Page Numbers: pp.14793-14796


We propose to overcome oxygen deficiency and light attenuation problems in photodynamic therapy (PDT), by separating photo-excitation and singlet oxygen delivery of the PDT process into two distinct operations to be carried out sequentially, at different locations. We now demonstrate the viability of this approach, using 2-pyridone derivative which yields a relatively stable endoperoxide. The initial storage endoperoxide obtained is transformed enzymatically into a more labile compound when placed in hypoxic cell cultures, and releases singlet oxygen significantly faster. The potential of this approach in advancing PDT beyond its current limits is exciting.