Aptamer-Conjugated Multifunctional Nanoflowers as a Platform for Targeting, Capture, and Detection in Laser Desorption Ionization Mass Spectrometry

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OCSOY I., Gulbakan B., SHUKOOR M. I., XIONG X., CHEN T., POWELL D. H., ...More

ACS NANO, vol.7, no.1, pp.417-427, 2013 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.1021/nn304458m
  • Journal Name: ACS NANO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.417-427
  • Open Archive Collection: AVESIS Open Access Collection
  • Hacettepe University Affiliated: No

Abstract

Although many different nanomaterials have been tested as substrates for laser desorption and ionization mass spectrometry (LOWS), this emerging field still requires more efficient multifuncional nanomaterials for targeting, enrichment, and detection. Here, we report the use of gold manganese oxide (Au@MnO) hybrid nanoflowers as an efficient matrix for LDI-MS. The nanoflowers were also functionalized with two different aptamers to target cancer cells and capture adenosine triphosphate (ATP). These nanoflowers were successfully used for metabolite extraction from cancer cell lysates. Thus, in one system, OUT multifunctional nanoflowers can (1) act as an ionization substrate for mass spectrometry, (2) target cancer cells, and (3) detect and analyze metabolites from cancer cells.