Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system


Bakhshpour M. , Chiodi E., Celebi I., SAYLAN İNCİ Y. , Unlu N. L. , Unlu M. S. , ...More

BIOSENSORS & BIOELECTRONICS, vol.201, 2022 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 201
  • Publication Date: 2022
  • Doi Number: 10.1016/j.bios.2021.113961
  • Title of Journal : BIOSENSORS & BIOELECTRONICS
  • Keywords: Immunoglobulin G, Interferometric reflecting imaging sensor, Medical applications, Real-time detection, HIGH-THROUGHPUT, LABEL-FREE, AFFINITY, BIOSENSOR, PLATFORM

Abstract

Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 mu g/mL with a low detection limit (0.25 mu g/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.