Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system

Bakhshpour M., Chiodi E., Celebi I., SAYLAN İNCİ Y., Unlu N. L., Unlu M. S., ...More

BIOSENSORS & BIOELECTRONICS, vol.201, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 201
  • Publication Date: 2022
  • Doi Number: 10.1016/j.bios.2021.113961
  • Journal Name: BIOSENSORS & BIOELECTRONICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Immunoglobulin G, Interferometric reflecting imaging sensor, Medical applications, Real-time detection, HIGH-THROUGHPUT, LABEL-FREE, AFFINITY, BIOSENSOR, PLATFORM
  • Hacettepe University Affiliated: Yes

Abstract

Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 mu g/mL with a low detection limit (0.25 mu g/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.