Real time monitoring and label free creatinine detection with artificial receptors


Topcu A. A. , ÖZGÜR E. , Yilmaz F., BERELİ N. , DENİZLİ A.

MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, vol.244, pp.6-11, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 244
  • Publication Date: 2019
  • Doi Number: 10.1016/j.mseb.2019.04.018
  • Title of Journal : MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
  • Page Numbers: pp.6-11

Abstract

Molecular imprinting technique is used to design artificial creatinine receptor on the gold surface of surface plasmon resonance (SPR) chip using N-methacryloyl-(L)-histidine methyl ester (MAH), as a functional monomer. Surface characterization of SPR sensor chip is performed with atomic force microscope (AFM), ellipsometry and contact angle measurements. Creatinine imprinted SPR sensor is characterized with a linear range between 1 and 100 mM and limit of detection (LOD) and limit of quantification (LOQ) of creatinine are found 57 mu M and 190 mu M, respectively. N-hydroxysuccinimide (NHS) and creatine molecules are selected to examine the selectivity of creatinine imprinted SPR sensor. Reusability studies of SPR sensor is determined in urine mimic samples.