Sol‐Gel Materials Used in Phosphoproteomics and Glycoproteomics Applications

Kayılı H. M., Atakay M., Salih B.

in: Recent Applications in Sol-Gel Synthesis, Usha Chandra, Editor, InTech, London, pp.213-230, 2017

  • Publication Type: Book Chapter / Chapter Vocational Book
  • Publication Date: 2017
  • Publisher: InTech
  • City: London
  • Page Numbers: pp.213-230
  • Editors: Usha Chandra, Editor
  • Hacettepe University Affiliated: Yes


post‐translational modifications (PTMs) of proteins. Both play key role in many vital biological

processes. Mass spectrometry is the most favored technique used for monitoring

the dynamics of PTMs. Mass spectrometric analysis of phosphopeptide and glycopeptide

is a crucial step in phosphoproteomics and glycoproteomics to understand the role of

these modifications in the cellular pathways. Enrichment and purification of (phosphoand

glyco‐) peptides and glycans are recommended prior to mass spectrometric analysis

because of the lower amount of modified peptides in a peptide mixture. Herein, we

present titanium/silica and zirconium/silica sol‐gel materials for the enrichment of (phospho‐

and glyco‐) peptides and glycans to enhance MALDI‐MS analysis performance.

Enrichment of (phospho‐ and glyco‐) peptides was successfully applied using standard

proteins including β‐casein, fetuin, and IgG as well as some complex medium. The solgel

materials were compared with commercial metal oxides regarding their modified

peptide enrichment performances.