in: Recent Applications in Sol-Gel Synthesis, Usha Chandra, Editor, InTech, London, pp.213-230, 2017
post‐translational modifications (PTMs) of proteins. Both play key role in many vital biological
processes. Mass spectrometry is the most favored technique used for monitoring
the dynamics of PTMs. Mass spectrometric analysis of phosphopeptide and glycopeptide
is a crucial step in phosphoproteomics and glycoproteomics to understand the role of
these modifications in the cellular pathways. Enrichment and purification of (phosphoand
glyco‐) peptides and glycans are recommended prior to mass spectrometric analysis
because of the lower amount of modified peptides in a peptide mixture. Herein, we
present titanium/silica and zirconium/silica sol‐gel materials for the enrichment of (phospho‐
and glyco‐) peptides and glycans to enhance MALDI‐MS analysis performance.
Enrichment of (phospho‐ and glyco‐) peptides was successfully applied using standard
proteins including β‐casein, fetuin, and IgG as well as some complex medium. The solgel
materials were compared with commercial metal oxides regarding their modified
peptide enrichment performances.