Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides

Bas D., BOYACI İ. H.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, cilt.400, sa.3, ss.703-707, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 400 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s00216-011-4827-4
  • Dergi Adı: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.703-707
  • Hacettepe Üniversitesi Adresli: Evet

Özet

A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R (2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.