Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides

Bas D., BOYACI İ. H.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol.400, no.3, pp.703-707, 2011 (SCI-Expanded) identifier identifier identifier


A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R (2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.