Research Information System
IEEE Sensors Journal, vol.26, no.9, pp.12889-12895, 2026 (SCI-Expanded, Scopus)
In the present study, ovalbumin imprinted surface plasmon resonance (SPR) sensor was developed for the sensitive and selective detection of ovalbumin. The surfaces of ovalbumin imprinted and non-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) SPR sensors were characterized by atomic force microscopy, contact angle measurements and fourier-transform infrared spectroscopy. Kinetic analyses were performed in phosphate buffer solution (pH 7.4) with ovalbumin concentrations ranging from 0.005 to 1.5 ng/mL. The detection limit in ovalbumin solutions was determined to be 0.00019 ng/mL. Selectivity studies demonstrated that the developed sensor exhibited higher affinity toward ovalbumin compared with lysozyme and transferrin. The relative selectivity coefficients of the ovalbumin-imprinted SPR sensor for ovalbumin/lysozyme and ovalbumin/transferrin were 5.94 and 8.05, respectively. This study also demonstrates that a high-molecular-weight protein such as ovalbumin can be successfully imprinted using the microcontact imprinting technique, providing a significant advancement in protein imprinting strategies. Therefore, the proposed SPR sensor offers a highly sensitive, selective, and reliable platform for the rapid detection of food allergens in complex matrices.