The main goal of the present study was to detect neopterin concentrations in human tear samples and to evaluate its potential correlation with serum neopterin levels. For this purpose, 20 systemically healthy volunteers were recruited, and both tear and serum samples were synchronically collected from each individual. Enzyme-linked immunoassay (ELISA) was carried out to detect the quantity of neopterin in the samples. Mean human tear neopterin levels were observed as 3 +/- 0.56 nM while mean serum neopterin was 9 +/- 1.25 nM. Additionally, a significant positive correlation between tear neopterin and serum neopterin concentrations was observed. This is the first report to show neopterin concentration in human tears as a biological sample. Collecting tears from the individuals is a non-invasive sampling method, and as an analytical aspect detection of neopterin by ELISA in tear samples construct a valuable, practical and cheap procedure for the diagnosis and monitoring of intraocular inflammation and systemic immune-mediated diseases.