Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+stem cells and stem cell-based decellularized matrix


Çankirili N., Kart D., Çelebi-Saltik B.

CELL AND TISSUE BANKING, cilt.21, sa.2, ss.215-231, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10561-020-09815-6
  • Dergi Adı: CELL AND TISSUE BANKING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CINAHL, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.215-231
  • Anahtar Kelimeler: CD146+stem cells, Umbilical cord, Biofilm, Decellularized matrix, Staphylococcus aureus, Pseudomonas aeruginosa, PERIVASCULAR CELLS, COLLAGEN-BINDING, IN-VIVO, PERICYTES, DIFFERENTIATION, FIBRONECTIN, ADHERENCE, LAMININ, CIPROFLOXACIN, ATTACHMENT
  • Hacettepe Üniversitesi Adresli: Evet

Özet

This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitro Pseudomonas aeruginosa and Staphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson's Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix proteins on biofilms were investigated on a drip flow biofilm reactor and their effects on gene expression were determined by RT-qPCR. We observed that CD146/105+ stem cells could differentiate adipogenically and decellularized matrix showed negative DAPI and positive collagen staining with Masson' s Trichrome. Proteome analysis of the decellularized matrix revealed some matrix components and growth factors. Although the decellularized matrix significantly reduced the cell counts of P. aeruginosa, no significant difference was observed for S. aureus cells in both groups. Supporting data was obtained from the gene expression results of P. aeruginosa with the significant down-regulation of rhlR and lasR. For S. aureus, icaADBC genes were significantly up-regulated when grown on the decellularized matrix.