Synthesis of novel substituted purine derivatives and identification of the cell death mechanism


Demir Z., Guven E. B. , ÖZBEY S. , Kazak C., Atalay R. C. , Tuncbilek M.

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, cilt.89, ss.701-720, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 89
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.ejmech.2014.10.080
  • Dergi Adı: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
  • Sayfa Sayıları: ss.701-720

Özet

Novel 9-(substituted amino/piperazinoethyl)adenines (4-12), 6-(substituted piperazino/amino)purines (15-27), 9-(p-toluenesulfonyl/cyclopentyl/ethoxycarbonylmethyl)-6-(substituted amino/piperazino)purines (28-34,36,37,38-41) were synthesized and evaluated initially for their cytotoxic activities on liver Huh7, breast T47D and colon HCT116 carcinoma cells. N-6-(4-Trifluoromethylphenyl)piperazine derivative (17) and its 9-(p-toluene-sulfonyI)/9-cyclopentyl analogues (28, 36) had promising cytotoxic activities. Compounds 17, 28 and 36 were further analysed for their cytotoxicity in a panel of a liver cancer cell lines. The compound 36 had better cytotoxic activities (IC50 <= 1 mu M) than the nucleobase 5-FU and nucleosides fludarabine, cladribine, and pentostatine on Huh7 cells. Cytotoxicity induced by 36 was later identified as senescence associated cell death by SA-beta-Gal assay. (C) 2014 Elsevier Masson SAS. All rights reserved.