In this study, it was aimed to examine the relationship between job characteristics of teaching profession (JCTP) and work-life balance of teachers (WLBT) according to the teachers' opinions. The study was conducted with the voluntary participation of 413 teachers working in official secondary education institutions in nine central districts of Ankara. The data of the study were collected with the Work-Life Balance Scale (WLBS) and the Job Characteristics of Teaching Profession Scale (JCTPS). In analyzing the obtained data, standard deviation, arithmetic mean, Pearson correlation, confirmatory factor analysis and multivariate regression analysis were used. As a result of the analyzes, it is observed that there is a significant correlation between the job characteristics of the teaching profession and the work-life balance of teachers. When evaluated in terms of the dimensions of the WLBS; there is a positive and significant relationship between the Work-Life Harmony dimension and the JCTP; although there is a negative and significant relationship between the Making Time for Personal Activities dimension and the JCTP. The results of multiple regression analysis point out that job characteristics of teaching profession is not a significant predictor for the work-life balance of teachers.