Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure
ADVANCED BIOLOGY, cilt.6, sa.6, 2022 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 6 Sayı: 6
- Basım Tarihi: 2022
- Doi Numarası: 10.1002/adbi.202101317
- Dergi Adı: ADVANCED BIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Anahtar Kelimeler: exosomes, extracellular vesicles, mesenchymal stem cells, metabolomics, neural differentiation, neural stem cells, subventricular zone, MARROW STROMAL CELLS, NEURONAL DIFFERENTIATION, SUBVENTRICULAR ZONE, NEUROTROPHIC FACTOR, PAX6 CONTROLS, EXOSOMES, VESICLES, PROTEIN, GROWTH, PROLIFERATION
- Hacettepe Üniversitesi Adresli: Evet
Özet
Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.