Comparison of the efficiency and relevancy of reference genes in crayfish tissue samples


ERGİN B. , PURALI N.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.41, ss.144-149, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 41 Konu: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1515/tjb-2016-0024
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Sayfa Sayıları: ss.144-149

Özet

Objective: Relevancy of the reference genes have not been investigated in crayfish tissue samples. Various genes have been proposed for establishing a reference level to analyze qPCR results obtained in different tissue samples. However, majority of those genes have been investigated solely in mammalian species. There is no report related to the relevancy and the efficiency of those common reference genes in crayfish tissue samples yet. The primary aim of the present work is to compare the expression level of a set of reference genes in various crayfish tissue samples to establish a reliable reference gene for quantitative expression level comparisons.