Ankaferd is a traditional folkloric medicine that has been used in Anatolia as a hemostatic agent for centuries. Ankaferd Blood Stopper (ABS) is comprised of a standardized plant extracts of T. vulgaris, G. glabra, V. vinifera, A.officinarum and U. Dioica. ABS modulates cellular apoptotic responses to hemorrhagic stress, as well as the hemostatic hemodynamic activity. Although the effects of ABS mainly depends upon the formation of an encapsulated protein network representing focal points for vital erythrocyte aggregation, integration of the functional proteomics, transcriptomics, and metabolomics will be important for detecting the exact 'mechanism-of-action' of ABS. In order to analyse the fourier transform infrared (FTIR) spectroscopic and mass spectrometric metabolomics, we prepared two-dimensional protein samples and used a Tensor 27 FTIR spectrometer, equipped with a high throughput extension (HTS-XT) accessory. The derivative spectra of metabolomic content of ABS and mass spectrometric and FTIR results were demonstrated. Biological fatty acids such as octanoic acid, heptanoic acid, decanoic acid, eicosanoic acid, octadecanoic acid, hexadecanoic acid, and others have been detected in the metabolomics of ABS. Our results about mass spectrometry and FTIR spectroscopy analyses ABS content within the many crossroads of hemostasis, infection, and neoplasia. Metabolomics studies may shed further light and represent a novel starting point on that perspective for the new avenues of ABS.