Purpose: The purpose of this study is to test the reliability of the grading system of standard fixation preference testing compared with the logarithmic scale of the minimum angle of resolution (logMAR) interocular visual acuity difference of patients with manifest strabismus and to test the strength of association between the interocular visual acuity difference and the grade of alternation. Methods: A total of 111 strabismic patients with large angle heterotropias (> 10 PD) were included in this study. The visual acuities of these patients were determined using the Snellen's, tumbling E, or Lea visual acuity charts as appropriate for the child's age and ability to cooperate. The results were converted to their logarithmic equivalents of minimum angle of resolution or recognition. Fixation preference was graded from 0 to 4 and was evaluated by observing the time-period through which fixation was maintained with the nonpreferred eye. Results: The median value of interocular visual acuity difference for grade 4 was determined as 0.0, grade 3 as 0.097, grade 2 as 0.17, grade 1 as 0.3, and grade 0 as 0.8. The grade of fixation preference was found to be inversely related to the logMAR interocular visual acuity difference (P < .0001). There was a significant difference between every fixation grade except grade 1 and grade 2 (P < .005). Conclusions. The grades used in fixation preference testing correlate with the logMAR visual acuity differences in strabismic amblyopic patients. However, the grading system does not imply a linear function in terms of visual acuity difference. The difference between the logMAR values of patients with grade 0 and 1 are more profound compared with other grades.