Effect of granulocyte-colony stimulating factor on spinal cord tissue after experimental contusion injury

Sanli A. M. , Serbes G., ÇALIŞKAN M., Kaptanoglu E., SARGON M. F. , Kilinc K., ...More

JOURNAL OF CLINICAL NEUROSCIENCE, vol.17, no.12, pp.1548-1552, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 12
  • Publication Date: 2010
  • Doi Number: 10.1016/j.jocn.2010.03.043
  • Page Numbers: pp.1548-1552


The purpose of this study was to investigate the early effects of granulocyte-colony stimulating factor (G-CSF) on myeloperoxidase (MPO) activity, lipid peroxidation (LPO) and ultrastructural findings in rats after spinal cord injury (SCI). We also compared the effects of G-CSF and methylprednisolone sodium succinate (MPSS). Wistar rats were divided into four groups: control. SCI alone (50 g/cm weight drop trauma), SCI + MPSS (30 mg/kg), and SCI + G-CSF (50 mu g/kg). Administration of G-CSF and MPSS significantly decreased LPO (p < 0.05) and MPO activity (p < 0.05) in the first 24 hours. MPSS was more effective than G-CSF in reducing LPO (p < 0.05) and in minimizing ultrastructure changes. The results of this study indicate that G-CSF exerts a beneficial effect by decreasing MPO activity and LPO and may reduce tissue damage in the first 24 hours after SCI. Our findings do not exclude the possibility that G-CSF has a protective effect on spinal cord ultrastructure after the first 24 hours following SCI. (C) 2010 Elsevier Ltd. All rights reserved.