Effect of granulocyte-colony stimulating factor on spinal cord tissue after experimental contusion injury

Sanli A. M., Serbes G., ÇALIŞKAN M., Kaptanoglu E., SARGON M. F., Kilinc K., ...More

JOURNAL OF CLINICAL NEUROSCIENCE, vol.17, no.12, pp.1548-1552, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 12
  • Publication Date: 2010
  • Doi Number: 10.1016/j.jocn.2010.03.043
  • Journal Name: JOURNAL OF CLINICAL NEUROSCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1548-1552
  • Hacettepe University Affiliated: Yes

Abstract

The purpose of this study was to investigate the early effects of granulocyte-colony stimulating factor (G-CSF) on myeloperoxidase (MPO) activity, lipid peroxidation (LPO) and ultrastructural findings in rats after spinal cord injury (SCI). We also compared the effects of G-CSF and methylprednisolone sodium succinate (MPSS). Wistar rats were divided into four groups: control. SCI alone (50 g/cm weight drop trauma), SCI + MPSS (30 mg/kg), and SCI + G-CSF (50 mu g/kg). Administration of G-CSF and MPSS significantly decreased LPO (p < 0.05) and MPO activity (p < 0.05) in the first 24 hours. MPSS was more effective than G-CSF in reducing LPO (p < 0.05) and in minimizing ultrastructure changes. The results of this study indicate that G-CSF exerts a beneficial effect by decreasing MPO activity and LPO and may reduce tissue damage in the first 24 hours after SCI. Our findings do not exclude the possibility that G-CSF has a protective effect on spinal cord ultrastructure after the first 24 hours following SCI. (C) 2010 Elsevier Ltd. All rights reserved.