Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

KURALAY F., ERDEM GÜRSAN K. A., ABACI S., Ozyoruk H., Yildiz A.

JOURNAL OF APPLIED ELECTROCHEMISTRY, vol.40, no.11, pp.2039-2050, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 11
  • Publication Date: 2010
  • Doi Number: 10.1007/s10800-010-0185-8
  • Journal Name: JOURNAL OF APPLIED ELECTROCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2039-2050
  • Hacettepe University Affiliated: Yes

Abstract

Poly(vinylferrocenium) (PVF+) modified gold (Au) electrode was developed in this study for the electrochemical sensing of deoxyribonucleic acid (DNA) hybridization based on the oxidation signals of polymer and guanine, and also for the electrochemical investigation of interaction of anticancer drug, mitomycin C (MC) and DNA immobilized onto PVF+ modified Au electrode. PVF+ modified Au electrode was prepared by electrooxidation of poly(vinylferrocene) PVF at +0.7 V versus Ag/AgCl reference electrode. The polymer modified electrode and DNA immobilized polymer modified electrode were characterized by X-ray photoelectron (XPS), Fourier transform infrared-attenuated total reflentance (FTIR-ATR) and alternating current (AC) impedance spectroscopy. For application studies, differential pulse voltammetry (DPV) technique was used.