Human serum albumin depletion based on dye ligand affinity chromatography via magnetic microcryogels

Ozbek M. A., BERELİ N., ÖZGÜR E., DENİZLİ A.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, cilt.34, ss.449-462, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/09205063.2022.2127142
  • Dergi Adı: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.449-462
  • Anahtar Kelimeler: magnetic microcryogel, dye ligand affinity chromatography, albumin depletion, proteomics, CRYOGELS, PROTEIN, PURIFICATION, ADSORPTION, STRATEGIES, SEPARATION, PHASE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

One of the primary purposes of proteomic studies is to analyze the proteins in the blood to be considered as biomarkers. Albumin, which constitutes the majority of total serum proteins, complicates the discovery of low-density proteins that are important for the diagnosis of diseases. Based on this, an alternative approach for albumin depletion was developed in this study by covalently attached Cibacron Blue 3GA (CB) to magnetic microcryogels. After detailed characterization of CB attached magnetic microcryogels synthesized via a microstencil array chip, albumin adsorption studies were performed to examine the optimum depletion conditions. In the presented study, the maximum albumin adsorption capacity (Q(max)) was calculated as 149.25 mg/mL in pH 5.0 acetate buffer solution, which is the optimum pH value for albumin. Experimental studies have demonstrated that CB-attached magnetic microcryogels can be reused without loss of performance for albumin depletion after 10 adsorption-desorption cycles.