One of the fastest growing fields in science in the 21st century is biotechnology. Biotechnology affects many areas of human life. Biotechnologically developed countries are also advanced in scientific literacy. Biotechnology literacy is an integral part of scientific literacy. It is believed that the greatest role in the development of scientific literacy and biotechnology literacy in societies belongs to science teachers. Therefore, the biotechnological literacy levels of prospective science teachers, who have a very effective role in creating the biotechnological literacy of students in our country, are wondered. It is aimed via this study to reveal how biotechnology literacy dimensions of prospective science teachers are and how biotechnology knowledge of prospective science teachers is. To reveal these, a biotechnology literacy test prepared in accordance with the test development process was used. Survey research method is preferred among quantitative research methods in this study. The prospective science teachers who were senior students at seven universities were chosen as a sample group of research. After application of test, It is revealed that most of the participants have low level for both biotechnology literacy dimension and biotechnology knowledge. Moreover, lots of misconceptions and misunderstandings were observed related to genetics and biotechnology among participants.