Calculus courses and the topics like differentiation and integral in these courses are important for secondary mathematics teacher candidates. Because these courses construct both a basis for the following mathematics courses and cover the topics which preservice teachers will teach when they became a teacher. In addition, the visual preferences of preservice teachers are closely related to the teaching methods they use. The aim of this study is to determine the visual preferences of preservice teachers in the content of integral and to determine the relationship between the teaching anxiety about mathematics. Furthermore the effects of grade level and GPA scores on mathematics teaching anxiety and visual preferences of preservice mathematics teachers will be determined. The study was carried out in 2012-2013 academic year with preservice mathematics teachers grade level 2-5 in a university in Turkey. (C) 2014 The Authors. Published by Elsevier Ltd.