Evaluation of a multi-parameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise


Orhan H., Van Holland B., Krab B., Moeken J., Vermeulen N., Hollander P., ...Daha Fazla

FREE RADICAL RESEARCH, cilt.38, sa.12, ss.1269-1279, 2004 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 12
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1080/10715760400013763
  • Dergi Adı: FREE RADICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1269-1279
  • Hacettepe Üniversitesi Adresli: Hayır

Özet

The objective of the present. study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6 +/- 0.7) exercised 60 min at 70% of maximal O-2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise. As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD).