Evaluation of a multi-parameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise

Orhan H., Van Holland B., Krab B., Moeken J., Vermeulen N., Hollander P., ...More

FREE RADICAL RESEARCH, vol.38, no.12, pp.1269-1279, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 12
  • Publication Date: 2004
  • Doi Number: 10.1080/10715760400013763
  • Title of Journal : FREE RADICAL RESEARCH
  • Page Numbers: pp.1269-1279


The objective of the present. study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6 +/- 0.7) exercised 60 min at 70% of maximal O-2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise. As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD).