Evaluation of a multi-parameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise

Orhan H., Van Holland B., Krab B., Moeken J., Vermeulen N., Hollander P., ...Daha Fazla

FREE RADICAL RESEARCH, cilt.38, ss.1269-1279, 2004 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 38 Konu: 12
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1080/10715760400013763
  • Dergi Adı: FREE RADICAL RESEARCH
  • Sayfa Sayıları: ss.1269-1279

Özet

The objective of the present. study was to evaluate a comprehensive set of urinary biomarkers for oxidative damage to lipids, proteins and DNA, in man. Eighteen moderately trained males (mean age 24.6 +/- 0.7) exercised 60 min at 70% of maximal O-2 uptake on a cycle ergometer. Urine fractions for 12 h were collected 1 day before, and for 3 consecutive days after exercise. As biomarkers of lipid peroxidation, 8 aldehydes (i.e. propanal, butanal, pentanal, hexanal, heptanal, octanal, nonanal and malondialdehyde-MDA)and acetone were analyzed in urines by gas chromatography with electron capture detection (GC-ECD).