Research Information System
3rd Balkan Symposium on Vegetables and Potatoes, Bursa, Turkey, 6 - 10 September 2006, pp.133-135
The influence of cold-shock treatment of Capsicum annuum L. buds at 4 degrees C for 48 h or 96 h, the additions of different concentrations of silver nitrate (AgNO3) (0, 1, 5 and 10 mg L-1) and 0.25% activated charcoal to the culture medium on pepper androgenesis were evaluated. Floral buds were collected whose anthers contained uninucleate microspores. Murashige and Skoog (MS) medium supplemented with combinations of 4 mg L-1 naphthalene acetic acid (NAA) + 1 mg L-1 benzyladenine (BA) or 1 mg L-1 NAA + 4 mg L-1 BA 0.8 % (w/v) agar and 3% (w/v) sucrose. The highest numbers of embryos (12.5 embryos/ 100 anthers) were obtained from the control anthers which were not subjected to cold treatments and cultured on MS medium containing 4 mg L-1 NAA and 1 mg L-1 BA and activated charcoal without AgNO3.