Effects of silver nitrate, activated charcoal and cold treatment on the in vitro androgenesis of pepper (Capsicum annuum L.)


Ozkum D., Tipirdamaz R.

3rd Balkan Symposium on Vegetables and Potatoes, Bursa, Turkey, 6 - 10 September 2006, pp.133-135 identifier identifier

Abstract

The influence of cold-shock treatment of Capsicum annuum L. buds at 4 degrees C for 48 h or 96 h, the additions of different concentrations of silver nitrate (AgNO3) (0, 1, 5 and 10 mg L-1) and 0.25% activated charcoal to the culture medium on pepper androgenesis were evaluated. Floral buds were collected whose anthers contained uninucleate microspores. Murashige and Skoog (MS) medium supplemented with combinations of 4 mg L-1 naphthalene acetic acid (NAA) + 1 mg L-1 benzyladenine (BA) or 1 mg L-1 NAA + 4 mg L-1 BA 0.8 % (w/v) agar and 3% (w/v) sucrose. The highest numbers of embryos (12.5 embryos/ 100 anthers) were obtained from the control anthers which were not subjected to cold treatments and cultured on MS medium containing 4 mg L-1 NAA and 1 mg L-1 BA and activated charcoal without AgNO3.