Determination of Embryotoxic Effects of In Ovo Administered Propofol on Peripheral Blood Alpha Naphthyl Acetate Esterase and Acid Phosphatase-Positive Lymphocytes


İzgi M., Sur E., Öznurlu Y., Özaydın T., Kuşat T.

Turkish Journal of Anaesthesiology and Reanimation, cilt.51, sa.1, ss.27-42, 2023 (Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.5152/tjar.2023.22726
  • Dergi Adı: Turkish Journal of Anaesthesiology and Reanimation
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.27-42
  • Anahtar Kelimeler: Alpha naphthyl acetate esterase, acid phosphatase, chicken embryo, enzyme histochemical methods, propofol
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Objective: The aim of this study is to determine the possible embryotoxic effects of propofol, a general anaesthetic agent that is commonly used in clinical practice, on peripheral blood lymphocytes using enzyme histochemical techniques. Methods: For this purpose, 430 laying hen fertile eggs were used for this study. The eggs were divided into 5 groups as control, solvent-control (saline), 2.5 mg kg−1 propofol, 12.5 mg kg−1 propofol, and 37.5 mg kg−1 propofol, and injections were performed via the air sac just before the incubation. The peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios were determined on the hatching day. Results: No statistically significant difference was found between both alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios of the control and solvent-control groups. However, when compared with the control and solvent-control groups, statistically significant decreases were observed in the peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios of the chicks from the propofol-injected groups. Besides, the difference between 2.5 mg kg−1 and 12.5 mg kg−1 propofol groups is not significant, whereas the difference between these 2 groups and the 37.5 mg kg−1 propofol group was statistically significant (P < .05). Conclusions: It was concluded that propofol given to fertilised chicken eggs just before incubation caused significant decreases in both the peripheral blood alpha naphthyl acetate esterase and acid phosphatase-positive lymphocyte ratios.