A highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5'-TA GGG CCA CTT GGA CCT-(CH2)(3)-SH-3' single-stranded probe (ss-DNA), 5'-AGG TCC AAG TGG CCC TA-3'(target DNA), 5'-SH-C-6-TAG GGC CA-3'(non-complementary-1) and 5'-SH-C-6-TGC CCG TTA CG 3-' (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (R-ct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detectionrange was from 1.0 x 10(-13) M to 1.0 x 10(-7) M and the detection limit was 1.10 x 10(-14) M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. (C) 2013 Elsevier B. V. All rights reserved.