Hybridoma cells immobilized on nonwoven polyester fabric discs: Proliferation and monoclonal antibody production in stationary culture

Aslankaraoglu E., Gumuderelioglu M., Gurhan S.

Journal of Biomaterials Applications, vol.18, no.2, pp.137-148, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1177/088532803038452
  • Journal Name: Journal of Biomaterials Applications
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.137-148
  • Hacettepe University Affiliated: Yes


Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immuno-sorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.