Hybridoma cells immobilized on nonwoven polyester fabric discs: Proliferation and monoclonal antibody production in stationary culture


Aslankaraoglu E., Gumuderelioglu M. , Gurhan S.

Journal of Biomaterials Applications, cilt.18, ss.137-148, 2003 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 18 Konu: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1177/088532803038452
  • Dergi Adı: Journal of Biomaterials Applications
  • Sayfa Sayıları: ss.137-148

Özet

Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immuno-sorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.