Hybridoma cells immobilized on nonwoven polyester fabric discs: Proliferation and monoclonal antibody production in stationary culture
Journal of Biomaterials Applications, vol.18, no.2, pp.137-148, 2003 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 18 Issue: 2
- Publication Date: 2003
- Doi Number: 10.1177/088532803038452
- Journal Name: Journal of Biomaterials Applications
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.137-148
- Hacettepe University Affiliated: Yes
Abstract
Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immuno-sorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.