This study has been carried out to determine the effect of self directed learning on student's success, self-directed learning readiness in laboratory, attitudes towards laboratory skills and chemistry laboratory anxiety in chemistry laboratory. Sampling group of the study is composed of 33, 5th grade students studying at Hacettepe University, Department of Secondary Science and Mathematics Education, Chemistry Education Programme. Research pattern of pretest post-test has been used in the research for one group. Self directed learning in laboratory has been planned according to the process of project based learning. "Chemistry Success Test", "Chemistry Laboratory Anxiety Scale", "Attitude towards Laboratory Skills Scale" and "Scale of Self-Directed Learning Readiness in Laboratory" have been used to collect data. Hypotheses of the study have been test using dependent sample t-test and multiple regression analysis. The results of the study have demonstrated that self-directed learning in laboratory has a meaningful contribution to chemistry success, self-directed learning readiness in laboratory and chemistry laboratory anxiety of students.