Age-related Changes in the Alkaline Phosphatase Activity of Healthy and Inflamed Human Dental Pulp

ASLANTAŞ E. E., Buzoglu H. D., Karapinar S. P., ÇEHRELİ Z. C., Muftuoglu S. F., ATİLLA P., ...Daha Fazla

JOURNAL OF ENDODONTICS, cilt.42, sa.1, ss.131-134, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.joen.2015.10.003
  • Dergi Adı: JOURNAL OF ENDODONTICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.131-134
  • Anahtar Kelimeler: Aging, alkaline phosphatase, biomineralization, dental pulp, pulp inflammation, CELLS, TEETH, EXPRESSION, DENTINOGENESIS, INFLAMMATION, COMPLEX, ABILITY
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Introduction: Alkaline phosphatase (ALP) plays an important role in inducing mineralization events in the dental pulp. This study investigated and compared the ALP levels in healthy and inflamed pulp in young and old human pulp. Methods: Tissue samples were collected from young (<30 years) and old (>60 years) donors. In both age groups, healthy human pulp (n = 18) were collected from extracted wisdom teeth. For reversible and irreversible pulpitis, pulp samples (n = 18 each) were obtained during endodontic treatment. ALP activity was assessed by spectrophotometry and immunhistochemistry. Results: Regardless of age, reversible pulpitis group samples showed a slight elevation in ALP activity compared with normal healthy pulp. In elderly patients, ALP expression with irreversible pulpitis was significantly higher than those with a healthy pulp (P < .05). Conclusions: In the hyperemic state, both the young and old pulp shows a slight increase in ALP activity, whereas in irreversible pulpitis, only the old pulp shows significantly elevated ALP levels. Such an increase may trigger calcification events, which may eventually cause difficulties in endodontic treatment procedures in elderly individuals.