Influence of a new nanoensemble of graphene oxide andInfluence of a new nanoensemble of graphene oxide and indole-3-acetic acid on the morphophysiological, biochemical, andindole-3-acetic acid on the morphophysiological, biochemical, and ultrastructural characteristics of upland cotton (Gossypiumultrastructural characteristics of upland cotton (Gossypium hirsutum L.)
Turkish Journal of Botany, cilt.50, sa.4, ss.318-340, 2026 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 50 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.55730/1300-008x.2908
- Dergi Adı: Turkish Journal of Botany
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Geobase, TR DİZİN (ULAKBİM), Academic Search Ultimate (EBSCO), Biomedical Reference Collection: Corporate Edition (EBSCO)
- Sayfa Sayıları: ss.318-340
- Hacettepe Üniversitesi Adresli: Evet
Özet
The exogenous application of phytohormones plays a crucial role in the activation of adaptive mechanisms in plants under both normal and stress conditions. Therefore, the present study investigates the synthesis, characterization, and application of nanoensembles based on indole-3-acetic acid (IAA) and graphene oxide (GO) nanolayers. The surface of GO synthesized by the modified Hummer method was noncovalently functionalized with IAA molecules. The structure and morphological properties of the prepared materials were analyzed by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Vis spectroscopy, and transmission electron microscopy. The IAA and IAA+GO nanoensemble enhanced the morphological parameters, lateral root formation, catalase and peroxidase activities, and nitric oxide production of upland cotton (Gossypium hirsutum L.). It also promoted stem elongation more effectively than pure IAA. Additionally, it significantly boosted polyphenol oxidase activity in leaf tissues. However, IAA+GO reduced the primary root length compared to IAA alone at the same concentration. In summary, the IAA+GO nanoensemble offers a promising approach for enhancing plant growth and development. Its impact on stem elongation, lateral root development, and the activation of enzymatic defense systems points to its potential application in agricultural practices aimed at improving crop productivity and stress resilience. Light and ultrastructural microscopy demonstrated that both IAA and IAA+GO induced cellular gigantism across all examined vegetative organs of cotton plants. Overall, the IAA+GO nanoensemble exhibits a synergistic effect on plant stem elongation and antioxidant defense mechanisms, enhancing nitric oxide-mediated signaling without inducing osmotic stress.