The purpose of this study is to investigate the frequency of hepatitis B (HBV), hepatitis C (HCV) and hepatitis G (HGV) virus, the demographical characteristics and clinical information of patients with beta-thalassemia major who receive frequent blood transfusion. Seventy patients with beta-thalassemia major [36 (51.5%) male, 34 (48.5%) female] are included in our study. The age of the patients was between two and 24 years. Age, gender and clinical information (time of first transfusion, number of transfusions and hepatitis B vaccination) of the patients are recorded. HBsAg, anti-HBs, anti-HBc IgG, HBV-DNA, anti-HCV, HCV-RNA and HGV-RNA tested in all patients. Three (4.3%) patients tested HGV-RNA positive. HBsAg in 1 (1.4%) patient, anti-HBs in 66 (94.3%) patients, anti-HBc IgG in 23 (% 32.9) patients, anti-HCV in14 (20%) patients and in 5 (7.1%) HCV-RNA tested positive. There was no relationship between HGV-RNA positivity and factors such as age, gender, number of transfusion, time of first transfusion, serum ferritin or serum ALT levels. While average serum ALT levels of two patients with both HGV-RNA and HCV-RNA positive was higher than the other patients. In 4.3% of the patients who received frequent blood transfusion the HGV-RNA was detected. While HGV infection can co-exist with HBV and HCV infections, however, when it exists alone, pronounced increase in transaminase levels had not occured.