Modulating Effects of Pycnogenol (R) on Oxidative Stress and DNA Damage Induced by Sepsis in Rats

Taner G., Aydin S., BACANLI M., Sarigol Z., Sahin T., BAŞARAN A. N., ...Daha Fazla

PHYTOTHERAPY RESEARCH, cilt.28, sa.11, ss.1692-1700, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 11
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1002/ptr.5184
  • Dergi Adı: PHYTOTHERAPY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1692-1700
  • Anahtar Kelimeler: Sepsis, Pycnogenol, DNA damage, Alkaline comet assay, Fpg-modified comet assay, PINE BARK EXTRACT, ANTIOXIDANT ACTIVITY, MATRIX METALLOPROTEINASES, COMET ASSAY, PROCYANIDINS, INHIBITION, FLAVONOIDS, MELATONIN, PROTECTS, MOLECULE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The aim of this study was to evaluate the protective effects of Pycnogenol (R) (Pyc), a complex plant extract from the bark of French maritime pine, on oxidative stress parameters (superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and total glutathione (GSH) and malondialdehyde (MDA) levels), an inflammatory cytokine (tumor necrosis factor alpha (TNF-) level) and also DNA damage in Wistar albino rats. Rats were treated with 100mg/kg intraperitonally Pyc following the induction of sepsis by cecal ligation and puncture. The decreases in MDA levels and increases in GSH levels, and SOD and GPx activities were observed in the livers and kidneys of Pyc-treated septic rats. Plasma TNF- level was found to be decreased in the Pyc-treated septic rats. In the lymphocytes, kidney, and liver tissue cells of the sepsis-induced rats, Pyc treatment significantly decreased the DNA damage and oxidative base damage using standard alkaline assay and formamidopyrimidine DNA glycosylase-modified comet assay, respectively. In conclusion, Pyc treatment might have a role in the prevention of sepsis-induced oxidative damage not only by decreasing DNA damage but also increasing the antioxidant status and DNA repair capacity in rats. Copyright (c) 2014 John Wiley & Sons, Ltd.