Electrochemical Studies on the Interaction between (E)-4-((6-Methoxybenzo[d]thiazol-2-yl)diazenyl)-N-Phenylaniline (MBDPA) and Bovine Serum Albumin


Surucu O. , ABACI S.

CHEMISTRYSELECT, cilt.2, ss.707-713, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 2 Konu: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1002/slct.201601657
  • Dergi Adı: CHEMISTRYSELECT
  • Sayfa Sayıları: ss.707-713

Özet

Protein electrochemistry is important in the understanding of the redox properties of biomacromolecules and in the development of commercial applications such as biosensors and diagnostic tools. For this purpose, the interaction between a novel synthesized (E)-4-((6-methoxybenzo[d]thiazol-2-yl)diazenyl)-N-phenylaniline and bovine serum albumin was investigated electrochemically. The measurements were carried out by using cyclic voltammetry technique and pencil graphite electrode. The modification of pencil graphite electrode was performed using (E)-4-((6-methoxybenzo[d]thiazol-2-yl)diazenyl)-N-phenylaniline. 10 cycles were made for coating. The interaction occurred with bovine serum albumin after 15 minutes. The characterization of surfaces was carried out using scanning electron microscope and electrochemical impedance spectroscopy techniques. The limit of detection and kinetic parameters were calculated using various concentrations of bovine serum albumin and various scan rates. The results were compared with hemoglobin and the priority of interaction between azo dyes and protein was proved. The binding constant and binding ratio were calculated as 3.98 x 10(22) and 1:5, respectively. The interference study was made using coexisting substances including metal ions, amino acids and carbohydrates in blood.