Bioguided fractionation of Polygonum alpinum and isolation and structure elucidation of active compounds


Demirezer L. Ö., KURUUZUM-UZ A., GUVENALP Z., SULEYMAN H.

PHARMACEUTICAL BIOLOGY, vol.44, no.6, pp.462-466, 2006 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2006
  • Doi Number: 10.1080/13880200600798544
  • Journal Name: PHARMACEUTICAL BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.462-466
  • Keywords: anti-inflammatory activity, flavonol glycosides, Polygonaceae, Polygonum alpinum, radical scavenging effect, FLAVONOIDS, LAPATHIFOLIUM, SENEGALENSE, HYDROPIPER, GLYCOSIDES, CHALCONE, NODOSUM, SSP
  • Hacettepe University Affiliated: Yes

Abstract

Bioguided fractionation of the methanol extract of aerial parts from Polygonum alpinum L. ( Polygonaceae) was investigated using the exudation phase of inflammation in aseptic arthritis model, which is produced by carrageenan and radical-scavenging properties. Flavonoid-containing fractions showed anti-inflammatory and radical-scavenging activities. Therefore, isolation and structure elucidation of flavonoids were carried out. The eight flavonol glycosides, which were quercetin 3-O-arabinofuranoside (avicularin) (1), quercetin 3-O-beta-glucuronopyranoside (2), quercetin 3-O-alpha-rhamnopyranosyl (1 -> 6)-beta-glucopyranoside (3), quercetin 3-O-beta-galacturonopyranoside (4), quercetin 3-O-beta-glucopyranoside (5), kaempferol 3-O-beta-galactopyranoside (6), quercetin 3-O-beta-galactopyranoside (hyperoside) (7), and myricetin 3-O-beta-galactopyranoside (8), were isolated from the methanol herb extract of Polygonum alpinum. The structures were established by spectroscopic methods.